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Technická 5
166 28 Prague 6 – Dejvice
IČO: 60461373 / VAT: CZ60461373

Czech Post certified digital mail code: sp4j9ch

Copyright: UCT Prague 2017
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REPARES (Research Platform on Antibiotic Resistance Spread through Wastewater Treatment Plants), a consortium of leading academic, industry, and policymakers knowledgeable in the Antibiotic Resistance (AR) subject, is geared towards contributing excellence in research on the spread of AR in wastewater treatment plants (WWTPs).

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 ◳ 1 (png) → (ořez 215*215px)

 

The REPARES Team, mostly made up of academic leaders from top universities across Europe, has established strong cooperation with leading European innovators and internationally renowned experts in the water-related antibiotic resistance field.

 

  • UCT will coordinate and work with AR experts to provide methodologies and development of the REPARES platform
  • UCP will leverage its expert leadership in wastewater-mediated antibiotic resistance.
  • TUDelft will deliver methods to apply for international projects successfully.
  • Wetsus will propel cooperation with the non-academic sector.
  • AAU will integrate the REPARES database within the world-accepted MiDAS.
  • UW will do the bioinformatics and the validation of qPCR primers.

 

Background

Antibiotics were developed in 1940 solely for the treatment of infections in humans and animals. Over the years, diseases considered deadly were considered non-issues due to the effectiveness of these medicines. Regrettably, the benefits of antibiotics have been hampered by the development of resistance to antibiotics by bacteria. In recent times certain antibiotics used to eradicate some bacteria have become inactive. Antibiotic resistance (AR) has become prevalent, spreading primarily in populations and the environment. Thus, the development and proliferation of antibiotic resistance are classified among major global threats by the World Health Organization ( ). In addition, the European Centre for Disease Prevention and Control described the colossal effect of AR on the human population resulting in approximately 25,000 deaths per annum, costing the health care sector over  1.5 billion every year.

Proper sanitation of the water cycle is essential to sustain environmental and human health. The role of wastewater treatment plants (WWTPs) and their microbiomes as putative hotspots for the proliferation of antibiotic resistance has questioned how effective these treatment systems are. Increasingly rising public, stakeholders, and governmental perception on this issue has highlighted the need for new quality criteria and installation upgrades.

Despite the gravity of the AR problem, in the wastewater sector, methodological biases have been the sole limiting factor on adequately monitoring the emission, fate, and possible amplification or removal of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) across WWTPs. Indeed, there is an urgent need to develop standardized methods and a well-curated database for accurate identification, characterization, and quantification of antibiotic resistance in such complex water matrices as wastewater. These issues are what REPARES aims to solve during the project period.

 ◳ 2 (png) → (ořez 215*215px)REPARES Goals

REPARES aims at advancing the European community’s know-how on antibiotic resistance across sanitation waterways, which in the future will bring society closer to achieving the Sustainable Development Goal of the United Nations. REPARES will act as an important vector to disseminate information on antimicrobial resistance spread within European WWTPs beyond science and technology to reach the academic and non-academic communities utilizing open events, popularization publications, and operation of the REPARES web platform.

Duration of Project

1 October 2019 - 30 September 2022

The relevance of research on antibiotic resistance

  • Antibiotics are one of the common contaminants in wastewater.
  • The increased bacterial antibiotic resistance stems from the spread of antibiotic-resistant bacteria and the transfer of resistance genes.
  • Antibiotic resistance can be acquired through random gene mutations and/or by horizontal gene transfer.
  • The World Health Organization qualified antibiotic resistance development as a significant global threat to society.

REPARES Objectives ◳ Untitled design (jpg) → (originál)

  • The increasing level of UCT’s scientific excellence
  • Improving scientific profiles of UCT’s researchers
  • Increasing UCT’s ability to apply for international projects successfully
  • Fostering cooperation with the non-academic sector
  • Advancing European community’s know-how on antibiotic resistance occurrence and spread in Europe
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Webpage under serious reconstruction! Be back soon!

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Webpage under serious reconstruction! Be back soon!

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Webpage under serious reconstruction! Be back soon!

Newsletter 1 (May 2020)

Newsletter 2 (November 2020)

Newsletter 3 (March 2021)

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 ◳ Graphical abstract (png) → (originál)

Do you think that when you get rid of bacteria in the toilet, it’s over?

Wrong! You send them to a party to make new friends, share experiences, food, and genetic information. Let's see together what is going on at such a party and how it relates to us. Can antibiotics stop all the bacteria? Certainly not all of them, the unstoppable are called superbugs.

Let's see what are these antibiotic-resistant bacteria and what can we do to avoid them...

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Research platform on antibiotic resistance spread through wastewater treatment plants

 ◳ Bartacek 5 (jpg) → (ořez 215*215px)

 

Jan Bartáček

Project Coordinator, UCT Prague

repares@vscht.cz

 ◳ Pokorna Lucie 6 (jpg) → (ořez 215*215px)

 

Lucie Pokorná

Project Administration, Webmaster

repares@vscht.cz

 ◳ Ojobe Lois (jpg) → (ořez 215*215px)

 

Bukola Lois Ojobe

Webmaster

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DATA


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    [nazev] => Interlaboratory Study
    [seo_title] => Interlaboratory Study
    [seo_desc] => 
    [autor] => Sabina Purkrtova
    [autor_email] => 
    [perex] => 

The methods for the quantification of ARGs enable quantifying ARGs amount in different parts of wastewater treatment plants (WWTPs), including inputs and outputs. This data allows to survey the fate of ARGs in WWTPs, to study the effect of single processes in WWTP on ARGs abundance, and last but not least to quantify the applied load of ARGs carried by WWTP outputs to the environments.

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 ◳ Join REPARES 2 (png) → (originál)The quantitative-based PCR methods (qPCR) belong to the most often used methods for quantifying ARGs, widely used in scientific studies. Despite this fact, the standard internationally validated or recommended methodology for qPCR quantification in WWTP samples has not yet been established. The quantitative standard methodology (“golden standard”) would cover not only the description of the single procedure steps but also the evaluation of test characteristics, for example, the selectivity (inclusivity/exclusivity), lowest validated level with satisfactory precision, repeatability, reproducibility and the uncertainty of the method.

In REPARES Ring test interlaboratory studies, we work on establishing such qPCR-based standard protocol and on evaluating the effect of single steps on its result.  These single steps cover the isolation of DNA and absolute qPCR quantification based on DNA intercalating dyes and using gBlocks.

At this moment, these interlaboratory studies are four sides, conducted by UCP Porto, UCT Prague, TU Delft, and Wetsus. The University of Warsaw ensures the bioinformatics support for these studies.

In the first run in 2020, we analyzed qPCR quantification of four different genes in activated sludges. All laboratories analyzed as the sample of activated sludges, as the sample DNA, isolated from it. The results imply that qPCR analyses have more extent effect on these ARGs quantification than performing the isolation of DNA.

In the second run, planned for September 2021, we will test these qPCR protocols for samples of anaerobic sludges and treated WWTP effluent and some other genes. The important part of the investigation will also direct to discover and quantify the impact of single qPCR steps (e.g., primers design, used master mixes, and chemicals).

We want to invite also other laboratories to join our interlaboratory studies.

We are looking for cooperating laboratories quantifying ARGs. It enables us to fortify these protocols verification (by testing our protocol if you perform qPCR quantification too) and/or to compare these protocols to other ARGs quantification methods (by comparing results if you perform different qPCR protocols or any other DNA based quantification methods).

If you are interested in joining us, please write to repares@vscht.cz subject: RING TEST. We will find a way how to cooperate.

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UCT Prague
Technická 5
166 28 Prague 6 – Dejvice
IČO: 60461373 / VAT: CZ60461373

Czech Post certified digital mail code: sp4j9ch

Copyright: UCT Prague 2017
Information provided by the Department of International Relations and the Department of R&D. Technical support by the Computing Centre.
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