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Technická 5
166 28 Prague 6 – Dejvice
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Czech Post certified digital mail code: sp4j9ch

Copyright: UCT Prague 2017
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REPARES (Research Platform on Antibiotic Resistance Spread through Wastewater Treatment Plants), a consortium of leading academic, industry, and policymakers knowledgeable in the Antibiotic Resistance (AR) subject, is geared towards contributing excellence in research on the spread of AR in wastewater treatment plants (WWTPs).

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 ◳ 1 (png) → (ořez 215*215px)

 

The REPARES Team, mostly made up of academic leaders from top universities across Europe, has established strong cooperation with leading European innovators and internationally renowned experts in the water-related antibiotic resistance field.

 

  • UCT will coordinate and work with AR experts to provide methodologies and development of the REPARES platform
  • UCP will leverage its expert leadership in wastewater-mediated antibiotic resistance.
  • TUDelft will deliver methods to apply for international projects successfully.
  • Wetsus will propel cooperation with the non-academic sector.
  • AAU will integrate the REPARES database within the world-accepted MiDAS.
  • UW will do the bioinformatics and the validation of qPCR primers.

 

Background

Antibiotics were developed in 1940 solely for the treatment of infections in humans and animals. Over the years, diseases considered deadly were considered non-issues due to the effectiveness of these medicines. Regrettably, the benefits of antibiotics have been hampered by the development of resistance to antibiotics by bacteria. In recent times certain antibiotics used to eradicate some bacteria have become inactive. Antibiotic resistance (AR) has become prevalent, spreading primarily in populations and the environment. Thus, the development and proliferation of antibiotic resistance are classified among major global threats by the World Health Organization ( ). In addition, the European Centre for Disease Prevention and Control described the colossal effect of AR on the human population resulting in approximately 25,000 deaths per annum, costing the health care sector over  1.5 billion every year.

Proper sanitation of the water cycle is essential to sustain environmental and human health. The role of wastewater treatment plants (WWTPs) and their microbiomes as putative hotspots for the proliferation of antibiotic resistance has questioned how effective these treatment systems are. Increasingly rising public, stakeholders, and governmental perception on this issue has highlighted the need for new quality criteria and installation upgrades.

Despite the gravity of the AR problem, in the wastewater sector, methodological biases have been the sole limiting factor on adequately monitoring the emission, fate, and possible amplification or removal of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) across WWTPs. Indeed, there is an urgent need to develop standardized methods and a well-curated database for accurate identification, characterization, and quantification of antibiotic resistance in such complex water matrices as wastewater. These issues are what REPARES aims to solve during the project period.

 ◳ 2 (png) → (ořez 215*215px)REPARES Goals

REPARES aims at advancing the European community’s know-how on antibiotic resistance across sanitation waterways, which in the future will bring society closer to achieving the Sustainable Development Goal of the United Nations. REPARES will act as an important vector to disseminate information on antimicrobial resistance spread within European WWTPs beyond science and technology to reach the academic and non-academic communities utilizing open events, popularization publications, and operation of the REPARES web platform.

Duration of Project

1 October 2019 - 30 September 2022

The relevance of research on antibiotic resistance

  • Antibiotics are one of the common contaminants in wastewater.
  • The increased bacterial antibiotic resistance stems from the spread of antibiotic-resistant bacteria and the transfer of resistance genes.
  • Antibiotic resistance can be acquired through random gene mutations and/or by horizontal gene transfer.
  • The World Health Organization qualified antibiotic resistance development as a significant global threat to society.

REPARES Objectives ◳ Untitled design (jpg) → (originál)

  • The increasing level of UCT’s scientific excellence
  • Improving scientific profiles of UCT’s researchers
  • Increasing UCT’s ability to apply for international projects successfully
  • Fostering cooperation with the non-academic sector
  • Advancing European community’s know-how on antibiotic resistance occurrence and spread in Europe
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Webpage under serious reconstruction! Be back soon!

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Webpage under serious reconstruction! Be back soon!

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Webpage under serious reconstruction! Be back soon!

Newsletter 1 (May 2020)

Newsletter 2 (November 2020)

Newsletter 3 (March 2021)

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 ◳ Graphical abstract (png) → (originál)

Do you think that when you get rid of bacteria in the toilet, it’s over?

Wrong! You send them to a party to make new friends, share experiences, food, and genetic information. Let's see together what is going on at such a party and how it relates to us. Can antibiotics stop all the bacteria? Certainly not all of them, the unstoppable are called superbugs.

Let's see what are these antibiotic-resistant bacteria and what can we do to avoid them...

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Research platform on antibiotic resistance spread through wastewater treatment plants

 ◳ Bartacek 5 (jpg) → (ořez 215*215px)

 

Jan Bartáček

Project Coordinator, UCT Prague

repares@vscht.cz

 ◳ Pokorna Lucie 6 (jpg) → (ořez 215*215px)

 

Lucie Pokorná

Project Administration, Webmaster

repares@vscht.cz

 ◳ Ojobe Lois (jpg) → (ořez 215*215px)

 

Bukola Lois Ojobe

Webmaster

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REPARES Events

Other Events

Coming soon:

15. International Conference on Antibiotics, Environment and Antibiotic Resistance

June 10-11, 2021 in Barcelona, Spain

15. International Conference on Novel Antibiotics from Actinomycetes and Antibiotic Resistance

June 14-15, 2021 in Montreal, Canada

4th International Caparica Conference in Antibiotic Resistance 2021

June 14- 17,  2021 in Caparica, Portugal

15. International Conference on Antimicrobial Plant Extracts, Phytochemicals and Antibiotic Resistance

June 17-18, 2021 in Riga, Latvia

JPIAMR Junior Researchers Online Workshop

29-30 June 2021

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A Part of REPARES secondment internships, researchers get involved in learning and contributing to understanding antibiotic resistance in the environment (wastewater) and finding solutions to stop or reduce its progression. 

In this event, UCT, Prague, Czech Republic, and WetsusLeeuwarden, Netherlands, met again where Jan Bartacek (UCT), Lucia Hernandez-Leal (Wetsus), Dana Vejmelkova (UCT), Bukola Lois Ojobe (UCT), Sam Rutten (Wetsus), and Veerle Levering (Wetsus) met to discuss the collaborative opportunities for both groups in the subset of greywater treatment and recycling.  

Bukola ‘Lois’ Ojobe, 3rd Ph.D. student working on removing pollutants in greywater, will be moving to Wetsus for a six-month internship working with Sam Rutten, a 2nd year Ph.D. student, and Veerle Levering, a masters student focusing on the effective use of nanofiltration for greywater recycling. 

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Seminar on the methods for ARB detection organized by REPARES proceeded for three days from 25.1. to 27.1.2021 (10:00-12:45). On Monday (25.1.) we focused on the phenotyping cultivation methods for the detection of ARB. The introductory lecture about these methods was given by dr. Sabina Purkrtová (UCT Prague). Consequently, Assoc. Prof. Lucia Bírošová (the Slovak University of Technology in Bratislava) demonstrated their use for the study of ARB in wastewater in her lecture brilliantly. An extremely interesting discussion followed these lectures, in which discussion we were able to welcome also Assoc. Prof. Helena Žemličková (NIPH CZ - National Institute for Health Protection). On Tuesday (26.1.) we continued with lectures on MALDI-TOF MS usage for the identification of microorganisms and the detection of SNP mutations in ARG. In these lectures dr. Sabina Purkrtová (UCT Prague) explained the principles and presented her practical laboratory experiences and protocols. The third and last day, Wednesday (27.1.). was devoted to MALDI-TOF MS again, but this time to its usage as the tool for the detection of antibiotic resistance mechanisms. Prof. Jaroslav Hrabák (Biomedical Center, Faculty of Medicine in Pilsen, Charles University) presented in his interesting and very broad lecture the history, development, principles, benefits, and limits of these applications. Ing. Milada Šolcová (UCT Prague) then presented her laboratory experience with MALDI-TOF MS detection of beta-lactamase activity. The very deep and vivid discussion then completed this seminar.

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Resistomap is organizing a series of live webinars from August to November 2020.
In the webinars, world-leading researchers and experts will offer insights based on their work on antibiotic resistance in the environment and discuss best strategies to avoid the further emergence and spread of antibiotic resistance.

‍Introduction to the environmental dimension of antibiotic resistance

‍Tuesday, 25 August 2020, 16:00 - 17:30 (EEST)
Speakers:
• Marko Virta (University of Helsinki, Finland)
• Ikuro Kasuga (University of Tokyo, Japan; VNU-Vietnam Japan University, Vietnam)
Registration link:
https://www.bigmarker.com/resistomap/august‍

Upcoming Webinar

Antibiotic resistance in water environments
‍Tuesday, 29 September 2020, 16:00 - 17:30 (EEST)
‍Antibiotic resistance in agroecosystem
‍Tuesday, 27 October 2020, 16:00 - 17:30 (EEST)

‍World Antibiotic Awareness Week (WAAW) 2020

‍Daily event from 18-24 November 2020
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The consortium of academic partners met to discuss progress made so far, evaluating the first round of an interlaboratory study that was just completed and coordinated by Aleksandra Miłobędzka from UCT Prague. David Calderón-Franco provided an insightful overview of the results of the 1st REPARES Seminar held in TU Delft focused on how different physical lysis steps in DNA extraction can influence the microbial composition and ARGs profiles. Sabina Purkrtová provided insight on the possibilities for 2nd round of the interlaboratory study with the idea to include more laboratories within the EU. However, the consortium agreed that the proper data analysis and detailed guidelines are desired prior to the 2nd round. The need to tackle antibiotic resistance in wastewater, stopping its spread, was expressly stressed.
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Dana Vejmelkova is doing her short research visit (March-August 2020) at Wetsus, in collaboration with Lucia Hernandez Leal, Heike Schmitt, Rebeca Pallares-Vega, Eri van Heijnsbergen, and Bianca de Vries. Dana participates in the investigation of the spread of antimicrobial resistance in the Vecht river (part of the MEDUWA-Vecht(e) project). She evaluates the quantity of 24 antibiotic resistance genes in the catchment and determines the influence of WWTPs and agriculture.

“I am glad I joined the Wetsus team. Despite my visit happens during COVID-19 time, I am gaining many useful experience and skills. Next to the project meetings, we have weekly Antibiotic resistance group meetings where all participants share their progress and support each other. Wetsus also organizes many events and does its best to keep people (socially) connected (e.g. online “pub” quiz and workouts).

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Dear non-academic partner/John (please, insert the name of your potential partner or friend)

On behalf of EU-funded project REPARES, I would like to invite you to become part of our REPARES platform, which aims at advancing know-how on antimicrobial resistance in the urban water cycle.

Can we add you to our contact list?

The REPARES project aspires to bring excellence in research on the spread of antimicrobial resistance (AMR) in wastewater treatment plants through establishing cooperation between leading European innovators and internationally renowned experts of the water-related antibiotic resistance field. Currently, University of chemistry and technology in Prague, Universidade Catolica Portuguesa, Delft University of Technology, Aalborg University, and Wetsus, the European centre of excellence for sustainable water technology, are active members of this consortium. We want to grow and involve non-academic partners in the public and private sectors.

The REPARES platform – one of the main products of the REPARES project – will offer space for the exchange of know-how between academia, industry and public stakeholders, act as a melting pot for the creation of new ideas and projects, and facilitate education and public debate on the spread of antibiotic resistance. The main activities will include organizing seminars, workshops, and public events, forming inter-sectoral and interdisciplinary research consortia, and realizing joint ARG-related research on every level of complexity.

We would like to add you to our contact list, where you can get invitations to workshops, seminars, and other initiatives that are organized by (or in cooperation with) REPARES around the topic of AMR. For more information on these events, please visit our website http://repares.vscht.cz/. In addition, you may receive our regular newsletter.

Please let us know, whether you are interested in reacting to this email.

 

Kind regards,

On behalf of REPARES

 

 

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We would like to invite you to a conference organized within bilateral initiative shAMRock – shocking antimicrobial resistance. The conference will take place on 12th May 2020 at University of Chemistry and Technology Prague

 Wastewater treatment plants (WWTPs) are claimed to be hotspots for antimicrobial resistance and can be an important threat to human health. Antimicrobial resistance in WWTPs is even more dangerous as the resistance genes can be transferred from environmental bacteria to pathogens and create an even higher risk for the wellbeing of citizens. Therefore, scope of the conference is monitoring of antimicrobial resistance in wastewater treatment plants - approaches and methods, results and perspectives.

There is no registration fee

The authors cover their own costs (travel, accommodation, etc.) to attend the conference.

Procedure for Submission is described here https://tvp.vscht.cz/research/projects-grants/50582/50844?ecrof&jazyk=en#novinka_detail25803388051713.

Submissions are welcome from 1st March 2020 and must be received before 31st March 2020. The submitted abstracts will be peer-reviewed.

Notification of acceptance will be sent before 10th April 2020. At least one author of each selected paper must register and attend the conference to present the paper (poster or platform presentation).

 
  KEY DATES

1.03.2020 - Abstracts submission opening 
31.03.2020 - Deadline for abstracts submission
10.04.2020 - Notification of acceptance, registration opens 
30.04.2020 - Registration closes

If you have questions, please contact us via e-mail: milobeda@vscht.cz

 

Conference invitation

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Aleksandra Miłobędzka on 27th Jan 2020 started working with Célia M. Manaia, Ana Catarina Ferreira, Joana Silva, Ivone Vaz-Moreira, Jaqueline Rocha and Adrian Górecki at UCP Porto during short research visit „Developments on the monitoring of the antibiotic resistance and associated genetic elements in environmental samples“. Aleksandra will stay at UCP till the end of April, till that time she is going to optimize protocol for IS26 in activated sludge using qPCR, get familiar with UCP’s research groups and labs, collaborate in great, sunny atmosphere and enjoy Portuguese culture.

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On January 21-23, 2020, REPARES Seminar 1" on the DNA isolation took place in Delft, The Netherlands. 

The seminar was hosted by David Weissbrodt and Mark Van Loosdrech from the Faculty of Applied Sciences of TU Delft. The practical part was guided by David Calderon and Ben Abbas.

Invited speakers Bruno Bicudo Perez, Ron Dirks, and Thomas Abeel gave an excellent insight on antibiotic resistance in water matrices.

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On the 2nd and 3rd December  2019, REPARES WORKSHOP 2 on Methodologies for antibiotic resistance monitoring took place in Porto, Portugal.
The discussion was raised to define and select standard methods for wastewater treatments plants sampling to detect and quantify ARGs. The workshop was hosted by the  Faculty of Biotechnology of  Universidade Católica Portuguesa and Prof. Celia Manaia.
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On Tuesday, October 1, 2019, all 5 partners of the newly launched REPARES project have met at the UCT Prague for their Kick-off meeting. 

Members from UCT Prague, TU Delft, Aalborg University, Universidade Catolica Portuguesa and Wetsus company have met to discuss their point of view on antibiotic resistance in wastewater and successfully started their new Horizon 2020 Twinning project. 

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June 2021

15. International Conference on Antibiotics, Environment and Antibiotic Resistance

June 10-11, 2021 in Barcelona, Spain

15. International Conference on Novel Antibiotics from Actinomycetes and Antibiotic Resistance

June 14-15, 2021 in Montreal, Canada

4th International Caparica Conference in Antibiotic Resistance 2021

June 14- 17,  2021 in Caparica, Portugal

15. International Conference on Antimicrobial Plant Extracts, Phytochemicals and Antibiotic Resistance

June 17-18, 2021 in Riga, Latvia

15. International Conference on Antibiotic Resistance and Attitudes on Antimicrobials

June 17-18, 2021 in Riga, Latvia

15. International Conference on Antibiotic Resistance and Infectious Disease Epidemiology

June 17-18, 2021 in Riga, Latvia

15. International Conference on Antibiotic Resistance and Resistance Detection

June 17-18, 2021 in Riga, Latvia

JPIAMR Junior Researchers Online Workshop

29-30 June 2021

15. International Conference on Antibiotics and Antibiotic Resistance

June 29-30, 2021 in Dubai, United Arab Emirates

July 2021

15. International Conference on Narrow-Spectrum Antibiotics and Antibiotic Resistance

July 08-09, 2021 in Prague, Czechia

15. International Conference on Antibiotic Resistance and Mechanisms

July 19-20, 2021 in Copenhagen, Denmark

15. International Conference on Antibiotic Resistance and Antibiotics

July 22-23, 2021 in Tokyo, Japan

15. International Conference on Antibiotic Resistance and Infection Control

July 22-23, 2021 in Tokyo, Japan

15. International Conference on Antibiotic Resistance and Antibiotic Prevention

July 29-30, 2021 in Dubai, United Arab Emirates

15. International Conference on Antibiotic Resistance and Drug Utilization

July 29-30, 2021 in Dubai, United Arab Emirates

15. International Conference on Antibiotic Resistance and Pharmacy

July 29-30, 2021 in Dubai, United Arab Emirates

August 2021

15. International Conference on Antibiotic Resistance and Prevention

August 19-20, 2021 in Bangkok, Thailand

5th AMR Conference, novel antimicrobials & AMR Diagnostics

24–26 August 2021, Delivered digitally

15. International Conference on Narrow-Spectrum Antibiotics and Antimicrobial Resistance

August 30-31, 2021 in Moscow, Russia

September 2021

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October 2021

15. International Conference on Antimicrobial Plant Extracts, Phytochemicals and Cross-Resistance

October 18-19, 2021 in Dubai, United Arab Emirates

15. International Conference on Environment, Antibiotics and Antibiotic Resistance

October 18-19, 2021 in Dubai, United Arab Emirates

15. International Conference on Antibiotic Resistance and Clinical Bacteriology

October 21-22, 2021 in Athens, Greece

15. International Conference on Antibiotic Resistance and Knowledge on Antimicrobials

October 21-22, 2021 in Athens, Greece

November 2021

World Anti-Microbial Resistance Congress

8-9 November 2021, Washington DC, United States

15. International Conference on Broad-Spectrum Antibiotics and Antimicrobial Resistancehttps://waset.org/broad-spectrum-antibiotics-and-antimicrobial-resistance-conference-in-november-2021-in-rome

November 11-12, 2021 in Rome, Italy

December 2021

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January 2022

16. International Conference on Antibiotic Resistance

January 28-29, 2022 in Sydney, Australia

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